Functional parameters of the bivalwe mollusk ark shell (Anadara broughtonii) hemolymph under exposure to hypoxia

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  • Authors: A. Yu. Andreyeva, E. S. Kladchenko, T. A. Kukhareva, V. N. Rychkova
  • Affiliations:
    1. A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russian Federation
  • Issue: No. 3 (23) (2022)
  • Section: Experimental Hydrobiology
  • Pages: 23–32
  • Keywords: hypoxia, bivalves, hemolymph, hemocytes, reactive oxygen species
  • DOI: 10.21072/eco.2022.23.03
  • UDC: 594.133‒111.3:612.22
  • EDN: PDBZBS
  • Published: 2022-09-30
  • Views: 183 Downloads: 84
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Andreyeva A. Yu., Kladchenko E. S., Kukhareva T. A., Rychkova V. N. Functional parameters of the bivalwe mollusk ark shell (Anadara broughtonii) hemolymph under exposure to hypoxia. Proceedings of the T.I.Vyazemsky Karadag Scientific Station - Nature Reserve of the Russian Academy of Sciences, 2022, no. 3 (23), pp. 23-32. https://doi.org/10.21072/eco.2022.23.03

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Abstract

Investigation of negative consequences of significant climate change on various types of aquatic organisms is an actual area of physiological research. Among key factors directly influencing aquatic organisms inhabiting coastal waters of the World Ocean at cellular and molecular levels, hypoxia plays a major role. In the present work, we studied the effect of short-term (24 h) and long-term (72 h) hypoxia on hemolymph cellular composition hemocyte morphology, and intracellular reactive oxygen species (ROS) content in the commercial bivalve (Anadara broughtonii). Under the experiment in vivo, we showed that hypoxia is not associated with shifts in the hemolymph cellular composition of the ark shell, and also does not affect the morphology of hemocytes. At the same time, short-term hypoxia (24 h) lead to a decrease in the level of ROS production by hemocytes. Results of the present work indicate that ark shell possesses mechanisms that compensate oxygen deficiency; these mechanisms allow maintaining the efficacy of hemocyte cellular immune reactions by recovery of the level of spontaneous ROS production under prolonged hypoxia.

Keywords: hypoxia, bivalves, hemolymph, hemocytes, reactive oxygen species

Authors

A. Yu. Andreyeva

PhD, leading researcher

A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russian Federation
E. S. Kladchenko

researcher

A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russian Federation
T. A. Kukhareva

PhD, senior researcher

A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russian Federation
V. N. Rychkova

junior researcher

A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russian Federation

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Funding

Работа проводилась в рамках госзадания No 121102500161-4 «Закономерности организации иммунной системы промысловых гидробионтов и исследование влияния факторов внешней среды на функционирование их защитных систем».

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