Sex features of proxidant-antioxidant blood system of round goby Neogobius melanostomus (Pallas, 1814) from the Black sea and the sea of Azov

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  • Authors: T. B. Sigacheva
  • Affiliations:
    1. A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russian Federation
  • Issue: No. 1 (21) (2022)
  • Section: Biodiversity research and environmental monitoring
  • Pages: 27–32
  • Keywords: round goby, blood, antioxidant enzyme activities, oxidized protein levels, Black Sea, Sea of Azov
  • DOI: 10.21072/eco.2022.21.03
  • UDC: [597.556.31-111.1:542.943](262.5+262.54)
  • EDN: OYLBPM
  • Published: 2022-04-22
  • Views: 170 Downloads: 73
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Sigacheva T. B. Sex features of proxidant-antioxidant blood system of round goby Neogobius melanostomus (Pallas, 1814) from the Black sea and the sea of Azov. Proceedings of the T.I.Vyazemsky Karadag Scientific Station - Nature Reserve of the Russian Academy of Sciences, 2022, no. 1 (21), pp. 27-32. https://doi.org/10.21072/eco.2022.21.03

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Abstract

The analysis of indicators of the prooxidant-antioxidant system parameters (activity of catalase (CAT), superoxide dismutase (SOD), peroxidase (PER), glutathione reductase (GR), glutathione-S-transferase (GST) and oxidized protein levels) in the blood of round goby Neogobius melanostomus (Pallas, 1814) specimens of different sexes from the coastal regions of the Black Sea (Sevastopol) and the Sea of Azov was carried out. A decrease in the activity of CAT, SOD (by 1.3–2 times) and an increase in the oxidized protein levels in the blood of males from the Sevastopol waters in comparison with similar indicators of females were found. In fish from the Sea of Azov, the sex differences were weakly pronounced. Analysis of blood biomarkers in the fish specimens of different sexes from two seas showed a higher level of oxidized proteins and low activities of CAT, SOD and GST in the blood of round goby males from the Black Sea. The results obtained make it possible to recommend carrying out bioindication studies using prooxidant-antioxidant blood system parameters of round goby on the same sex specimens, preferably males.

Keywords: round goby, blood, antioxidant enzyme activities, oxidized protein levels, Black Sea, Sea of Azov

Authors

T. B. Sigacheva

PhD, senior researcher

A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russian Federation

References

Дубинина Е. Е., Бурмистов С. О., Ходов Д. А., Поротов И. Г. Окислительная модификация белков сыворотки крови человека, метод её определения // Вопросы медицинской химии. – 1995. – № 1. – С. 24–26.

Лакин Г. Ф. Биометрия. – 4-е изд., перераб. и доп. – Москва : Высш. шк., 1990. – 352 с.

Правдин И. Ф. Руководство по изучению рыб (преимущественно пресноводных) / под ред. П. А. Дрягина, В. В. Покровского. – 4-е изд., перераб. и доп. – Москва : Пищ. пром-сть, 1966. – 376 с.

Руднева И. И. Ответные реакции морских животных на антропогенное загрязнение Чёрного моря : дис. … д-ра биол. наук : 03.00.29. – Севастополь, 2000. – 329 c.

Руднева И. И., Скуратовская Е. Н. Половые особенности активности антиоксидантных ферментов крови некоторых прибрежных видов рыб Чёрного моря // Вопросы ихтиологии. – 2009. – Т. 49, № 1. – С. 125–128. – https://doi.org/10.1134/S0032945209010159

Суховская И. В., Смирнов Л. П., Немова Н. Н., Комов В. Т. Влияние ртути на фракционный состав низкомолекулярных пептидов мускулатуры речного окуня Perca fluviatilis // Вопросы ихтиологии. – 2001. – Т. 41, № 5. – С. 699–703.

Чеснокова И. И. Биомаркеры черноморских рыб как показатели экологического состояния среды их обитания : автореф. дис. … канд. биол. наук : 03.02.10. – Севастополь, 2017. – 22 с.

Chainy G. B. N., Samantaray S., Samanta L. Testosterone-induced changes in testicular antioxidant system // Andrologia. – 1997. – Vol. 29, iss. 6. – P. 343–349. – https://doi.org/10.1111/j.14390272.1997.tb00328.x

Huh K., Shin U. S., Choi J. W., Lee S. I. Effect of sex hormones on lipid peroxidation in rat liver // Archives of Pharmacal Research. – 1994. – Vol. 17, iss. 2. – P. 109-114. – https://doi.org/10.1007/BF02974233

Karadag H., Firat Ö., Firat Ö. Use of oxidative stress biomarkers in Cyprinus carpio L. for the evaluation of water pollution in Ataturk Dam Lake (Adiyaman, Turkey) // Bulletin of Environmental Contamination and Toxicology. – 2014. – Vol. 92, iss. 3. – P. 289–293. – https://doi.org/10.1007/s00128-013-1187-0

Kovyrshina T. B., Rudneva I. I. The Response of blood biomarkers of the round goby Neogobius melanostomus (Pallas, 1814) (Perciformes: Gobiidae) to chronic coastal pollution in the Sea of Azov // Russian Journal of Marine Biology. – 2018. – Vol. 44, № 4. – P. 328–333. – https://doi.org/10.1134/S1063074018040065

Pajović S. B., Saicić Z. S., Spasić M. B., Petrović V. M., Martinović J. V. Effects of progesterone and estradiol benzoate on glutathione dependent antioxidant enzyme activities in the brain of female rats // General Physiology and Biophysics. – 1999. – Vol. 18, iss. 1. – P. 35–44.

Parolini M., Iacobuzio R., Felice B., Bassano B., Pennati R., Saino N. Age- and sex-dependent variation in the activity of antioxidant enzymes in the brown trout (Salmo trutta) // Fish Physiology and Biochemistry. – 2019. – Vol. 45, iss. 1. – P. 145–154. – https://doi.org/10.1007/s10695-0180545-6

Persky A. M., Green P. S., Stubley L., Howell C. O., Zaulyanov L., Brazeau G. A., Simpkins J. W. Protective effect of estrogens against oxidative damage to heart and skeletal muscle in vivo and in vitro // Proceedings of the Society for Experimental Biology and Medicine. – 2000. – Vol. 223, iss. 1. – P. 59–66. – https://doi.org/10.1046/j.1525-1373.2000.22308.x

Winzer K., Winston G. W., Becker W., Van Noorden C. J. F., Kőehler A. Sex-related responses to oxidative stress in primary cultured hepatocytes of European flounder (Platichthys flesus L.) // Aquatic Toxicology. – 2001. – Vol. 52, iss. 2. – P. 143–155. – https://doi.org/10.1016/S0166445X(00)00137-5

Funding

Работа выполнена в рамках государственного задания ФИЦ ИнБЮМ по теме: «Закономерности формирования и антропогенная трансформация биоразнообразия и биоресурсов Азово-Черноморского бассейна и других районов Мирового океана», регистрационный No 121030100028-0.

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