The Effect of Inorganic Mercury on RPA1 and P53 Expression in the Brain of Rainbow Trout Oncorhynchus Mykiss

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  • Authors: E. V. Sukharenko1, V. S. Nedzvetsky2,3
  • Affiliations:
    1. Kerch State Maritime Technological University, Kerch, Russian Federation
    2. Bingöl University, Bingöl, Turkey
    3. Oles Honchor Dnipro National University, Dnipro, Ukraine
  • Issue: No. 3 (15) (2020)
  • Section: Experimental Hydrobiology
  • Pages: 81–91
  • Keywords: inorganic mercury, RPA1, p53, neurotoxicity, environment pollution, Rainbow trout
  • DOI: 10.21072/eco.2021.15.07
  • UDC: 57.021
  • EDN: KKGDLE
  • Published: 2020-09-18
  • Views: 154 Downloads: 70
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Sukharenko E. V., Nedzvetsky V. S. The Effect of Inorganic Mercury on RPA1 and P53 Expression in the Brain of Rainbow Trout Oncorhynchus Mykiss. Proceedings of the T.I.Vyazemsky Karadag Scientific Station - Nature Reserve of the Russian Academy of Sciences, 2020, no. 3 (15), pp. 81-91. https://doi.org/10.21072/eco.2021.15.07

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Abstract

The study was carried out with Rainbow troute exposed to inorganic mercury 25% LC50 and 50% LC50 for 2 and 7 days. Obtained results have shown that downregulation of p53 level accompanied with upregulation of RPA1 in the brain of fish treated with sublethal dose. Therefore, upregulation of RPA1 expression could be one of critic factor of cell adaptation in stressed with inorganic Hg fish brain. Taking together presented in our study results, we can assume the presence of interaction between Hg-induced ROS generation and the modulation of both p53 and RPA1 content during cellular response to mercury cytotoxicity in a rainbow trout brain. Presented results in our study are reported the first time that a lack of p53 and upregulation of RPA1 in fish brain after inorganic Hg exposure. The revealed RPA1 upregulation in fish brain could serve at least a part of cellular response to DNA breaks caused by inorganic Hg.

Keywords: inorganic mercury, RPA1, p53, neurotoxicity, environment pollution, Rainbow trout

Authors

E. V. Sukharenko

D. Sc., Assistant Professor, Professor

Kerch State Maritime Technological University, Kerch, Russian Federation
V. S. Nedzvetsky

D. Sc., Professor

Bingöl University, Bingöl, Turkey / Oles Honchor Dnipro National University, Dnipro, Ukraine

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