Genetic engineering in microalgae biotechnology: achievements and prospects
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Abstract
This review summarizes information on the application of genetic engineering methods for culturing diatoms and green microalgae. The basic methods, the results achieved and the prospects for the use of genetically modified microalgae strains in biotechnology are discussed. Successful examples of application of directed genome editing methods for modification of model species of microalgae Chlamydomonas reinhardtii P.A.Dangeard, 1888 and Phaeodactylum tricornutum Bohlin, 1897 are presented. It is shown that knockouts and overexpression of key genes allow to significantly increase lipid accumulation in algal biomass without significant effect on growth, demonstrating the promising potential of this approach in optimizing the biotechnological potential of strains.
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References
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